時間：2024年1月4日 14:00-16:00
地點：逸夫樓B139
主講人：吳輝 教授
報告摘要：A novel strategy to finely control a large metabolic flux by using a “metabolic transistor” approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The “metabolic transistor” strategy was applied to controlling electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The 4-hydroxybenzoate geranyltransferase (lePGT-1, encoded by the lepgt gene) from Lithospermum erythrorhizon can competitively consume substrates required for the biosynthesis of ubiquinone-8 (Q8). Therefore, flexible regulation of LA production can be achieved by controlling the expression level of the lepgt gene to precisely control the flux of the ETC. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy. This strategy was also applied to the regulation of the molar percentage (mol%) of the LA fraction in P(3HB-co-LA) biosynthesis.
主講人簡介：
吳輝，華東理工老員工物工程學院教授、博導。入選國家高層次青年人才，教育部“霍英東青年教師基金” 獲得者。華東理工大學發(fā)酵工程博士、萊斯老員工物工程系和中科院合成生物學重點實驗室博士后，主要從事微生物低碳代謝工程與代謝調控研究。主持國家自然科學基金面上/青年項目、國家重點研發(fā)計劃課題/子課題等項目。Biotechnol Adv、Metab Eng、ACS Sustain Chem Eng、Bioresource Technol、ACS Synth Biol等期刊發(fā)表論文60余篇，出版專著3部。曾先后獲寶鋼優(yōu)秀教師獎，侯德榜化工技術獎青年獎、上海市教學成果獎一等獎、中國石油與化工公司產(chǎn)品優(yōu)秀成果一等獎。目前擔任中國輕工業(yè)生物基材料工程重點實驗室副主任，中國生物工程學會青年工作委員會委員，中國生物工程學會一碳生物技術專委會委員，上海市生物工程學會一碳生物技術專委會副主任委員，上海市生物工程學會合成生物學委員會委員，生物反應器工程國家重點實驗室固定人員。Front Microbiol副主編、J Ind Microbiol Biot、J Chem Technol Biot、Front Bioeng Biotechnol編委和Bioresource Bioprocess青年編委。
